FIG 3.

Entry of pseudotyped viruses carrying SARS-CoV-2 S with single or multiple mutations on the RBD site of S protein into CRFK cells expressing ACE2 of various species. CRFK cells expressing ACE2 from different animal species were infected with pseudotyped viruses expressing single or multiple S protein substitutions. Following incubation of the cells for 48 h, cells were lysed, and relative luminescence units were measured. Each mutant pseudotyped virus was compared with the parental pseudotyped virus (PA), and data are presented as the fold change to PA. One-way ANOVAs on the log₁₀-transformed raw relative fluorescence units were used to compare the parental group and other groups. Statistical differences between mutation and the parental virus groups are indicated with an asterisk (*, P < 0.05). Red square: human data.