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. 2022 May 12;10(3):e00797-22. doi: 10.1128/spectrum.00797-22

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Copyright © 2022 Ham et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Allelic discrimination plots of SNP assays for Spike K417T, E484K, E484Q, and L452R. Synthetic RNA controls from six SARS-CoV-2 type strains were amplified in triplicate at 4 × 10⁴ genome copies/assay via TaqPath RT-qPCR along with no template controls. Synthetic RNA strains that failed to amplify on K417T, E484K, E484Q, or L452R assays lacked both targeted alleles. Sequenced positive saliva samples (n = 125) were loaded in duplicate to determine the detection range of the assay in saliva. Data were plotted by using the absolute fluorescence of each reporter dye probe.