FIG 3.

Application and interpretation of differential VOC assays. (A) VOC strain typing by mutation site. Each strain will produce a different combination of results from the six assays. Strains with an alternate allele at the mutation site will produce inconclusive results. BA.1 and BA.2 patterns were determined by publicly available strain data. All other strain results were validated with synthetic RNA. (B) Example of strain-typing workflow using minimal steps. Saliva samples that are determined positive by routine diagnostic testing can be analyzed with various assays that produce differential results for each VOC.