TABLE 1.

Performance of RT-qPCR deletion assays in saliva^a

Genome copies/RT-qPCR assay	Mean Cq values +/- SD
	SΔ69-70	ORF1aΔ3675-3677	K417T	E484K	E484Q	L452R
4 × 106	13.11 ± 0.17	12.68 ± 0.11	19.19 ± 0.06	19.12 ± 0.07	19.43 ± 0.07	19.47 ± 0.11
4 × 105	16.33 ± 0.04	16.21 ± 0.09	22.27± 0.27	22.10 ± 0.04	22.61 ± 0.02	21.20 ± 0.67
4 × 104	19.61 ± 0.10	19.40 ± 0.03	24.84 ± 0.57	25.25 ± 0.04	25.72 ± 0.08	24.71 ± 0.23
4 × 103	23.05 ± 0.04	22.80 ± 0.03	29.20 ± 0.86	28.56 ± 0.09	29.26 ± 0.10	27.60 ± 0.14
4 × 102	26.54 ± 0.11	26.23 ± 0.08	34.00 ± 1.07	32.23 ± 0.07	32.99 ± 0.10	31.16 ± 0.10
4 × 101	30.05 ± 0.15	29.32 ± 0.14	37.14 ± 1.58	35.24 ± 0.26	36.21 ± 0.11	34.48 ± 0.08
4 × 100	nd	32.35 ± 0.44	39.85 ± 0.83	38.56 ± 0.33	38.64 ± 0.15	37.06 ± 0.42
E	97.12%	101.44%	89.52%	102.19%	101.40%	112.04%
R2	0.9997	0.9995	0.9927	0.9994	0.9979	0.9953

^aLimits of detection are in bold.