TABLE 3.

Performance of Spike SNP assays in saliva^a

Assay	Screening assay result	Whole genome sequencing result			Assay clinical analysis
		Reference	Substitution	Alternate	% Accuracy	% Clinical sensitivity	% Clinical specificity	% PPV	% NPV
K417T	Reference	176	0	0	99.20 [98.10, 100.0a]	97.30 [93.55, 100.0]	100.0 [100.0]	100.0 [100.0]	98.88 [97.33, 100.0]
	Substitution	0	64	0
	Inconclusive	2	0	8
E484K	Reference	173	0	0	96.40 [94.09, 98.71]	88.31 [80.67, 95.95]	100.0 [100.0]	100.0 [100.0]	95.05 [91.90, 98.20]
	Substitution	0	68	0
	Inconclusive	2	7	0
E484Q	Reference	179	0	0	99.60 [98.88, 100.0]	98.59 [95.83, 100.0]	100.0 [100.0]	100.0 [100.0]	99.44 [98.35, 100.0]
	Substitution	0	0	0
	Inconclusive	1	0	70
L452R	Reference	181	2	0	96.80 [94.62, 98.98]	88.41 [80.38, 96.47]	100.0 [100.0]	100.0 [100.0]	95.77 [92.90, 98.64]
	Substitution	0	61	0
	Inconclusive	4	2	0

^aUpper-bound value cannot exceed 100.0%. This applies to all measurements. 95% confidence interval is represented in brackets.