FIG 1.

SIE dependence on primary and secondary MOIs. Experiments done in triplicate on PK15 cells. (A) Diagram of the timeline of experimentation. (B) Comparison of SIE across a 4 BY 4 matrix of primary and secondary infections. The top row corresponds to the MOI of the secondary virus (YFP labeled), while the vertical column corresponds to the MOI of the primary virus. Images were acquired and analyzed 7 to 9 h after initial infection (or 4 to 6 h after superinfection). Column with CFP fluorescent expression represents confluent infection with primary virus and is representative of all conditions in the row. (C) Analysis of SIE with CHX treatment. Experiments were repeated with previously described SIE conditions. CHX treatment occurred between the application of the primary and secondary inocula. The top row depicts fluorescent protein expression without CHX (MOI 10), while the middle (MOI 10) and the bottom (MOI 100) depict fluorescent protein expression with CHX treatment.