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. 2022 May 16;10(3):e00068-22. doi: 10.1128/spectrum.00068-22

FIG 3.

FIG 3

Ebselen suppresses RNA-dependent RNA polymerase (RdRP) synthesis in the deformed wing virus (DWV)-infected pupal head cells. (A) Effect of increasing concentrations of ebselen on RdRP synthesis in DWV-infected (+) pupal head cells. Dimethyl sulfoxide (DMSO) was used as the control. RdRP precursor with 3C protease (97 kDa) is indicated by a black arrowhead. This band was absent in DWV-uninfected (−) cells. The replicate gel was stained with Instant Blue to show that equal amount of protein was applied in each lane. Molecular weight (kDa) of the protein marker is indicated at the left. (B) Band intensity of the RdRP precursor was compared between DMSO and ebselen at the indicated concentrations by the Dunnett method (one-tailed). Mean values ± SD (error bars) are shown (n = 5). (C) Luminescence generated by the luciferase activity dependent on the intracellular ATP level was compared between the pupal head cells treated with DMSO or ebselen at the indicated concentrations by the Dunnett method (one-tailed). There was no significant difference between DMSO and 30 μM ebselen. Mean values ± SD (error bars) are shown (n = 5).