FIG 1.
S. thermophilus urea hydrolysis, growth rate, and homolactic fermentation in milk. Monitoring 13C urea consumption (A), acidification (B), lactose consumption (C), lactic acid production (D), cell density (E), and generation time (F) during the growth of S. thermophilus MIM13 in skimmed milk with (open circle) or without (black circle) supplementation of flurofamide as a urease inhibitor. The generation time (F) was calculated between 120 min and 150 min and between 135 min and 165 min. Error bars represent the standard deviation calculated on three experimental replicates.