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. 2022 Apr 25;10(3):e02760-21. doi: 10.1128/spectrum.02760-21

TABLE 2.

PFK activity

Sample PFK activitya (nmol/min/mL) % Activity increment using NH3 or NH4+ as PFK effectors
S. thermophilus EdC total protein extract 575 ± 166 NA
S. thermophilus EdC total protein extract, 0.5 mM NH3 1278 ± 193 122
S. thermophilus EdC total protein extract, 0.5 mM NH4Cl 2321 ± 332 304
Bacillus sp. PFK 8.4 ± 0.7 NA
Bacillus sp. PFK, 0.5 mM NH3 32 ± 9 281
Bacillus sp. PFK, 0.5 mM NH4Cl 58 ± 14 591
a

PFK activity is reported as nmol/min/mL = milliunit/mL. One unit of PFK is the amount of enzyme that will generate 1.0 μmol of NADH per minute at pH 7.4 at 37°C. NA, not applicable.