FIG 3.

Stability of atpT transcripts under different conditions. (A–E) Levels of atpT mRNA detected in wild-type Synechocystis 6803 using Northern blot hybridization. The cultures were incubated in the dark overnight to induce high atpT mRNA levels, rifampicin was added, and the cultivation continued under the indicated conditions for 60 min. 5S rRNA was used as a loading control. Note that different exposure times were used for the different experiments. (F) Signal intensities of atpT from panels (A–E) normalized for the respective 5S rRNA intensity plotted against the time after rifampicin addition. Quantity One software was used to quantify transcript signal intensities. The fitted decay curves are plotted as lines colored according to the respective conditions. Numerical values for half-lives in minutes and 95% confidence intervals (95% CI) are indicated. All stability analyses were repeated at least twice.