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. 2022 Jun 2;10(3):e01112-22. doi: 10.1128/spectrum.01112-22

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Copyright © 2022 Morogovsky et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — HGT of chromosomal cyp51A^R in the A. fumigatus CEA10 background. (A) Experimental outline and result demonstrating HGT between akuB^KU80-cyp51A^R donor and CEA10-hph recipient strains under VCZ+Hyg selection. (B) Conidial diameter of akuB^KU80-cyp51A^R and CEA10-hph parental strains and colonies 1 to 3 generated under VCZ+Hyg selection (****, P < 0.001). (C) Propidium iodide nuclear staining (left; representative image from flow cytometry analysis) and (D) flow cytometry analysis (right) of CEA10-hph parental strain (green line, mean intensity of 26,171 arbitrary units) and colony 1 (red line, mean intensity 53,212 arbitrary units), generated under VCZ+Hyg selection.