FIG 4.

HGT of the cyp51A^R plasmid in the A. fumigatus CEA10 background under VCZ stress alone. (A) Experimental outline and result demonstrating HGT between CEA17/pAMA-cyp51A^R donor and CEA10-hph recipient strains under either VCZ or VCZ+Hyg selection. (B) PCR analysis of three VCZ selection colonies (1 to 3) and three VCZ+Hyg selection colonies (4 to 6) with hph-SH1-specific primer pairs HPH_2 and HPH_3 confirms that they are in the CEA10-hph recipient strain background. (C) Plasmid rescue and restriction analysis confirm that the recipient colonies contain pAMA-cyp51A^R transferred from the CEA17/pAMA-cyp51A^R donor.