FIG 5.

HGT of the cyp51A^R plasmid in the A. fumigatus Af293 background under hypoxic stress alone. (A) Experimental outline and result demonstrating HGT between Af293.1/pAMA-cyp51A^R donor and Af293-hph recipient strains under hypoxic selection. (B) PCR analysis of three colonies (1 to 3) under VCZ+Hyg selection after HGT with hph-SH1-specific primer pairs HPH_2 and HPH_3 confirms that they are in the Af293-hph recipient strain background. (C) Plasmid rescue and restriction analysis confirm that recipient colonies 1 to 3 contain pAMA-cyp51A^R transferred from the Af293.1/pAMA-cyp51A^R donor.