FIG 5.

Effect of normalization of CA HIV DNA quantification in PBMCs. (A) Mean of nonnormalized dilution-factored CA HIV DNA quantification selected with the defined linearity criteria, as well as the median between assays are represented. (B) Normalization of the PBMC panel quantification with data obtained from the 8E5 cell panel (1e5 and 5e6 cells per vial) as well as cross-subtype normalization. The participants are color coded and the assays identified by a unique icon. qPCR is represented as circles, dPCR as triangles. (C) For nonnormalized and normalized data, the distance to the median, median absolute deviation, and the number of assays falling outside 0.20 of median are reported. Reduction of the distance to the median after normalization is highlighted in green and increase in orange. NA indicates that the calculation was not possible.