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. 2022 Jun 23;219(8):e20220323. doi: 10.1084/jem.20220323

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© 2022 Banach et al.

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Figure S5. — Screening of antibody multi-mutation variants by AlphaLISA, BLI, and ITC. (a) BLI affinity of improved CIS43 variants; Var2, Var10, Var11, and Var18 were determined against fl_PfCSP, NTDS_5/3_CSP, NPDP19, Pep21, Pep25, and Pep29. (b) Isothermal titration calorimetry of CSP_SA_mut with various improved CIS43 variants. The affinities and stoichiometry are shown for both KD1 and KD2, respectively. (c) Comparison of the protection capacity of L9, CIS43_Var10, D3, and combination mutant variants.