TABLE 1.
Growth of rhodococci on seawater-based medium containing the AF of Arabian light crude oila
| Species | Strain | Growth | Colony morphology |
|---|---|---|---|
| Rhodococcus australis | ATCC 35215 | − | R |
| Rhodococcus coprophilus | ATCC 29080 | − | R |
| Rhodococcus equi | IAM 3223 | − | R |
| Rhodococcus equi | IFO 14956 | − | S/M |
| Rhodococcus erythropolis | ATCC 19369 | + | R |
| Rhodococcus erythropolis | ATCC 27854 | − | S/R |
| Rhodococcus erythropolis | ATCC 47072 | − | S/R |
| Rhodococcus erythropolis | DSM 1069 | − | S/R |
| Rhodococcus erythropolis | IFO 15567 | − | S |
| Rhodococcus erythropolis | JCM 3201 | − | R |
| Rhodococcus fascians | IFO 15528 | − | R |
| Rhodococcus globerulus | ATCC 14346 | + | R |
| Rhodococcus globerulus | ATCC 14898 | − | S |
| Rhodococcus globerulus | ATCC 15076 | + | R |
| Rhodococcus globerulus | ATCC 15903 | − | S/R |
| Rhodococcus globerulus | ATCC 19370 | − | S |
| Rhodococcus globerulus | ATCC 21022 | ++ | S-M |
| Rhodococcus globerulus | ATCC 21292 | − | R |
| Rhodococcus globerulus | ATCC 21505 | − | S/R |
| Rhodococcus globerulus | ATCC 21506 | + | S/R |
| Rhodococcus globerulus | ATCC 21602 | − | S/R |
| Rhodococcus globerulus | ATCC 25669 | − | R |
| Rhodococcus globerulus | ATCC 25688 | − | R |
| Rhodococcus globerulus | ATCC 31130 | − | R |
| Rhodococcus globerulus | IFO 14531 | − | S/R |
| Rhodococcus opacus | ATCC 17039 | − | R |
| Rhodococcus opacus | ATCC 51881 | − | R |
| Rhodococcus opacus | ATCC 51882 | − | R |
| Rhodococcus percolatus | JCM 10087 | − | S/R |
| Rhodococcus rhodnii | ATCC 35071 | − | S |
| Rhodococcus rhodochrous | ATCC 12483 | − | S/R |
| Rhodococcus rhodochrous | ATCC 12674 | − | S/R |
| Rhodococcus rhodochrous | ATCC 13808 | − | R |
| Rhodococcus rhodochrous | ATCC 14341 | − | R |
| Rhodococcus rhodochrous | ATCC 14347 | − | S/R |
| Rhodococcus rhodochrous | ATCC 14348 | − | R |
| Rhodococcus rhodochrous | ATCC 14349 | − | R |
| Rhodococcus rhodochrous | ATCC 14350 | − | R |
| Rhodococcus rhodochrous | ATCC 15610 | − | R |
| Rhodococcus rhodochrous | ATCC 15905 | − | R |
| Rhodococcus rhodochrous | ATCC 15906 | − | R |
| Rhodococcus rhodochrous | ATCC 15998 | − | R |
| Rhodococcus rhodochrous | ATCC 17041 | + | M/R |
| Rhodococcus rhodochrous | ATCC 17043 | − | S |
| Rhodococcus rhodochrous | ATCC 17895 | − | R |
| Rhodococcus rhodochrous | ATCC 184 | − | S |
| Rhodococcus rhodochrous | ATCC 19067 | − | S/R |
| Rhodococcus rhodochrous | ATCC 19140 | − | M/R |
| Rhodococcus rhodochrous | ATCC 19149 | − | R |
| Rhodococcus rhodochrous | ATCC 19150 | − | S,M/R |
| Rhodococcus rhodochrous | ATCC 21197 | − | R |
| Rhodococcus rhodochrous | ATCC 21198 | − | R |
| Rhodococcus rhodochrous | ATCC 21199 | − | R |
| Rhodococcus rhodochrous | ATCC 21243 | + | R |
| Rhodococcus rhodochrous | ATCC 21291 | − | R |
| Rhodococcus rhodochrous | ATCC 21766 | + | R |
| Rhodococcus rhodochrous | ATCC 21785 | − | R |
| Rhodococcus rhodochrous | ATCC 21924 | − | R |
| Rhodococcus rhodochrous | ATCC 271 | − | R |
| Rhodococcus rhodochrous | ATCC 29670 | − | R |
| Rhodococcus rhodochrous | ATCC 29675 | − | R |
| Rhodococcus rhodochrous | ATCC 33025 | − | R |
| Rhodococcus rhodochrous | ATCC 33258 | ++ | M |
| Rhodococcus rhodochrous | ATCC 33278 | − | R |
| Rhodococcus rhodochrous | ATCC 4001 | − | R |
| Rhodococcus rhodochrous | ATCC 4004 | − | R |
| Rhodococcus rhodochrous | ATCC 4276 | − | R |
| Rhodococcus rhodochrous | ATCC 53968 | ++ | M |
| Rhodococcus rhodochrous | ATCC 9356 | − | S/R |
| Rhodococcus rhodochrous | ATCC 999 | − | R |
| Rhodococcus ruber | IFO 15591 | − | R |
| Rhodococcus zopfii | ATCC 51349 | − | R |
| Rhodococcus sp. | KL6 | ++ | M |
| Rhodococcus sp. | PR4 | ++ | M |
| Rhodococcus sp. | PG7-2 | ++ | M |
The AF was prepared by column chromatography by using Silica Gel C-200 (Wako Pure Chemicals) activated at 180°C for 20 h. Arabian crude oil dissolved in n-hexane at a concentration of 100 mg/ml was applied to the column, and the column was serially eluted with 3 bed volumes of n-hexane, benzene–n-hexane (1:1), and chloroform. The eluate obtained with benzene–n-hexane (1:1) was kept and used as the AF. The growth of rhodococci in SWYAF was examined at 30°C. M, mucoid; S, smooth; R, rough; R/M, a few rough colonies appeared among mucoidal colonies; R-M, rough sectors appeared in mucoidal colonies; ++, cultures became turbid; +, cultures became less turbid; cultures remained transparent and almost all of the oil adhered to the inner surfaces of tubes.