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. 2000 Nov;66(11):5083–5086. doi: 10.1128/aem.66.11.5083-5086.2000

TABLE 2.

Nucleotide sequences of primers used for PCR amplification of the genes orfA, mdrL, qacA, and smr

Gene Primera Sequence (5′ to 3′) Amplicon size (kb)
orfA orf1 AAATGATTGCTCGTGAAGCT 0.467
orf2 CGCACACCATTTTAATTCTG
mdrL lltb1 AAATGGATAACAGCGGCAG 1.136
lltb2 TGTAAGGTAAAATGTGCTGG
qacA qac3 ACTACTGATATGATGACATCA 1.512
qac4 AGTTATATCAAGTGATTTGGG
smr smr1 ATAGCCATAAGTACTGAAGTT 0.291
smr2 ACCGAAAATGTTTAACGAAAC
a

These primers were formulated from sequences deposited in the EMBL/GenBank databases under accession no. X56628 for the qacA gene (18), M37889 for the smr gene (12), and AJ009627 for the orfA and mdrL genes (8).