RFLP patterns of the reference strains and isolates. Reference strains: Sabin 1, 2, and 3, Mahoney, Lansing, and Saukett. Isolates: G17-21, G4-2, G3-1, O41-1, G27-14, G4-18, and G19-4. UC, uncut; MW, DNA molecular weight marker (MspI digest of pBR322). Strains G17-21, G3-1, and G4-18 had restriction patterns similar to those of Sabin strains. G4-2, O41-1, G27-14, and G19-4 had patterns different from those of Sabin strains.