Figure 3.
The balance between electron transport chain complexes sustains high mitochondrial membrane potential in HSC. Top: Schematic representation of the ETC complex in HSC and committed cells. ETC complexes I–IV transfer protons from the mitochondrial matrix to the periplasmic space to contribute to increase mitochondrial membrane potential (ΔΨmt). This proton-motive force of ΔΨmt is used and depolarized by F1FO ATP synthase (or complex V) to generate ATP. Unlike complex I and complex V, complex II expression is similar between HSCs and mature populations. This allows HSCs to sustain a high ΔΨmt, which cannot be dissipated by ATP synthase, since it is barely expressed. Bottom: Representative immunofluorescence images of NDUFV1 (ETC complex I), SDHA (ETC complex II) and ATP5A (ATP Synthase) in HSC and committed cells (Lin–). Mitochondrial membrane potential measured by TMRM in HSCs and Lin– cells. Data are modified from.59 Template created with BioRender.com. ATP = adenosine triphosphate; ETC = electron transport chain; HSC = hematopoietic stem cell; TMRM = tetramethylrhodamine methyl ester perchlorate.