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. 2022 Jun 16;18(6):e1010588. doi: 10.1371/journal.ppat.1010588

Fig 9. Identified pIIIa-host interactions are crucial across different HAdV species.

Fig 9

(A) Multiple sequence alignment of representative pIIIa amino acids across HAdV A-G species using BioEdit. Sequence conservation across types are color coded. (B) Co-immunoprecipitation of HAdV-D37 and HAdV-C5 infected cells (MOI of 1 and 5, respectively, 24 hpi) show pIIIa-USP9x and pIIIa-RANBP2 interactions for both viruses. (C) HAdV-C5 viral replication in USP9x and RANBP2-siRNA treated HEK293 cells at an MOI of 5 and 24 hpi. Data shown are mean ± standard deviation for 3 replicates (P>0.05 by unpaired t-test, two-tailed).