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. 2022 Jun 29;18(6):e1010628. doi: 10.1371/journal.ppat.1010628

Fig 3. Downregulation of ULF and SIVA1 proteins inhibits ubiquitination and degradation of p14ARF protein in H. pylori-infected cells.

Fig 3

(A) Analysis of p14ARF protein expression after downregulation of ULF (left panel), SIVA1 (central panel), or MKRN1 (right panel) with specific siRNAs in SNU1 cells co-cultured with H. pylori strains 7.13 or B128 for 4hrs or left uninfected. Transfections with scrambled siRNA (Scr siRNA) were used as controls (n = 3). (B) The same as (A), but SNU1 cells were transfected with plasmids expressing ULF (left panel) or SIVA1 (right panel) proteins. Transfections with empty vector (pcDNA3) were used as controls (n = 3). (C) Analysis of p14ARF protein ubiquitination after downregulation of ULF or SIVA1 with siRNAs in SNU1 cells co-cultured with H. pylori strain 7.13. Bottom panels show expression of ULF and SIVA1 in input cell lysates. All experiments were performed at least three times (n = 3) with representative blots shown. Graph panels depict the densitometric analyses. Protein expression levels in control cells were arbitrarily set at 1.