| Genetic reagent (D. melanogaster) |
ubi-P4M::GFP
|
This paper |
|
See “Materials and methods” |
| Genetic reagent (D. melanogaster) |
ubi-P4M × 2::GFP
|
This paper |
|
See “Materials and methods” |
| Genetic reagent (D. melanogaster) |
ubi-PLC-PH::GFP
|
This paper |
|
See “Materials and methods” |
| Genetic reagent (D. melanogaster) |
ubi-PLC-PH::RFP
|
This paper |
|
See “Materials and methods” |
| Genetic reagent(D. melanogaster) |
y[1] M{RFP[3xP3.PB] GFP[E.3xP3]=vas int.Dm}ZH-2A w[*]; PBac{y[+]-attP-9A}VK00022
|
Bloomington Drosophila Stock Center |
BDSC:24868; RRID:BDSC_24868
|
|
| Genetic reagent (D. melanogaster) |
y[1] w[*] P{y[+t7.7]=nos-phiC31\int.NLS}X; PBac{y[+]-attP-3B}VK00040
|
Bloomington Drosophila Stock Center |
BDSC:35568; RRID:BDSC_35568
|
|
| Genetic reagent (D. melanogaster) |
PI4K-2KD
|
This paper |
|
See “Materials and methods” |
| Genetic reagent (D. melanogaster) |
PI4K-3KD
|
This paper |
|
See “Materials and methods” |
| Genetic reagent (D. melanogaster) |
UAS-AT1.03NL1
|
DGRC#117,011 Tsuyama et al., 2017
|
DGRC#117,011 |
|
| Genetic reagent (D. melanogaster) |
Cy2-Gal4
|
Gift from David Bilder Queenan et al., 1997
|
|
|
| Genetic reagent (D. melanogaster) |
rbo::GFP
|
Gift from Kendal Broadie Huang et al., 2004
|
|
|
| Genetic reagent (D. melanogaster) |
lgl::mCherry
|
Dong et al., 2015
|
|
|
| Genetic reagent (D. melanogaster) |
lgl::GFP
|
Dong et al., 2015
|
|
|
| Genetic reagent (D. melanogaster) |
UAS-PI4KIIIα-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:35256; FLYB:FBst0035256; RRID:BDSC_35256
|
FlyBase symbol: P{TRiP.GL00144}attP2 |
| Genetic reagent (D. melanogaster) |
UAS-Rbo-RNAi
|
Vienna Drosophila Resource Center |
VDRC:v47751; FLYB:Bst0467525 |
FlyBase symbol: P{GD14013}v47751 |
| Genetic reagent (D. melanogaster) |
UAS-ttc7-RNAi
|
Vienna Drosophila Resource Center |
VDRC:v35881; FLYB: FBst0461391 |
FlyBase symbol: P{GD13893}v35881 |
| Genetic reagent (D. melanogaster) |
UAS-Plc21C-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:33719; FBst0033719 RRID:BDSC_33719
|
|
| Genetic reagent (D. melanogaster) |
UAS-pis-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:55602; FBst0055602; RRID:BDSC_55602
|
|
| Genetic reagent (D. melanogaster) |
UAS-PI4KIIIα-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:38242; FBst0038242; RRID:BDSC_38242
|
|
| Genetic reagent (D. melanogaster) |
UAS-synj-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:44420; FBst0044420; RRID:BDSC_44420
|
|
| Genetic reagent (D. melanogaster) |
UAS-rdgβ-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:44523; FBst0044523; RRID:BDSC_44523
|
|
| Genetic reagent (D. melanogaster) |
UAS-INPP5E-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:41701; FBst0041701; RRID:BDSC_41701
|
|
| Genetic reagent (D. melanogaster) |
UAS-CG5026-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:42759; FBst0042759; RRID:BDSC_42759
|
|
| Genetic reagent (D. melanogaster) |
UAS-Pten-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:33643; FBst0033643; RRID:BDSC_33643
|
|
| Genetic reagent (D. melanogaster) |
UAS-Pi3k21B-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:36810; FBst0036810; RRID:BDSC_36810
|
|
| Genetic reagent (D. melanogaster) |
UAS-fwd-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:35257; FBst0035257; RRID:BDSC_35257
|
|
| Genetic reagent (D. melanogaster) |
UAS-mtm-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:31552; FBst0031552; RRID:BDSC_31552
|
|
| Genetic reagent (D. melanogaster) |
UAS-PIP4K-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:35660; FBst0035660; RRID:BDSC_35660
|
|
| Genetic reagent (D. melanogaster) |
UAS-PI4KIIIα-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:35643; FBst0035643; RRID:BDSC_35643
|
|
| Genetic reagent (D. melanogaster) |
UAS-INPP5E-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:34037; FBst0034037; RRID:BDSC_34037
|
|
| Genetic reagent (D. melanogaster) |
UAS-CG3632-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:38341; FBst0038341; RRID:BDSC_38341
|
|
| Genetic reagent (D. melanogaster) |
UAS-mtm-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:38339; FBst0038339; RRID:BDSC_38339
|
|
| Genetic reagent (D. melanogaster) |
UAS-PIP4K-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:35338; FBst0035338; RRID:BDSC_35338
|
|
| Genetic reagent (D. melanogaster) |
UAS-INPP5E-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:34037; FBst0034037; RRID:BDSC_34037
|
|
| Genetic reagent (D. melanogaster) |
UAS-CG3632-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:38341; FBst0038341; RRID:BDSC_38341
|
|
| Genetic reagent (D. melanogaster) |
UAS-mtm-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:38339; FBst0038339; RRID:BDSC_38339
|
|
| Genetic reagent (D. melanogaster) |
UAS-PIP4K-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:35338; FBst0035338; RRID:BDSC_35338
|
|
| Genetic reagent (D. melanogaster) |
UAS-FIG4-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:38291; FBst0038291; RRID:BDSC_38291
|
|
| Genetic reagent (D. melanogaster) |
UAS-CG5026-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:38309; FBst0038309; RRID:BDSC_38309
|
|
| Genetic reagent (D. melanogaster) |
UAS-PI4KIIα-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:35278; FBst0035278; RRID:BDSC_35278
|
|
| Genetic reagent (D. melanogaster) |
UAS-norpA-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:31113; FBst0031113; RRID:BDSC_31113
|
|
| Genetic reagent (D. melanogaster) |
UAS-sktl-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:27715; FBst0027715; RRID:BDSC_27715
|
|
| Genetic reagent (D. melanogaster) |
UAS-fwd-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:29396; FBst0029396; RRID:BDSC_29396
|
|
| Genetic reagent (D. melanogaster) |
UAS-CG6707-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:28316; FBst0028316; RRID:BDSC_28316
|
|
| Genetic reagent (D. melanogaster) |
UAS-synj-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:34378; FBst0034378; RRID:BDSC_34378
|
|
| Genetic reagent (D. melanogaster) |
UAS-pis-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:29383; FBst0029383; RRID:BDSC_29383
|
|
| Genetic reagent (D. melanogaster) |
UAS-sl-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:32385; FBst0032385; RRID:BDSC_32385
|
|
| Genetic reagent (D. melanogaster) |
UAS-CG42271-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:29411; FBst0029411; RRID:BDSC_29411
|
|
| Genetic reagent (D. melanogaster) |
UAS-Plc21C-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:31270; FBst0031270; RRID:BDSC_31270
|
|
| Genetic reagent (D. melanogaster) |
UAS-Pi3K92E-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:35798; FBst0035798; RRID:BDSC_35798
|
|
| Genetic reagent (D. melanogaster) |
UAS-Pi3K59F-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:33384; FBst0033384; RRID:BDSC_33384
|
|
| Genetic reagent (D. melanogaster) |
UAS-sl-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:35604; FBst0035604; RRID:BDSC_35604
|
|
| Genetic reagent (D. melanogaster) |
UAS-CG9784-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:34723; FBst0034723; RRID:BDSC_34723
|
|
| Genetic reagent (D. melanogaster) |
UAS-Pi3K68D-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:34621; FBst0034621; RRID:BDSC_34621
|
|
| Genetic reagent (D. melanogaster) |
UAS-Ocrl-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:34722; FBst0034722; RRID:BDSC_34722
|
|
| Genetic reagent (D. melanogaster) |
UAS-sktl-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:35198; FBst0035198; RRID:BDSC_35198
|
|
| Genetic reagent (D. melanogaster) |
UAS-Pten-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:25841; FBst0025841; RRID:BDSC_25841
|
|
| Genetic reagent (D. melanogaster) |
UAS-CG33981,fab1-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:35793; FBst0035793; RRID:BDSC_35793
|
|
| Genetic reagent (D. melanogaster) |
UAS-Plc21C-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:32438; FBst0032438; RRID:BDSC_32438
|
|
| Genetic reagent (D. melanogaster) |
UAS-rdgB-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:28796; FBst0028796; RRID:BDSC_28796
|
|
| Genetic reagent (D. melanogaster) |
UAS-CG3530-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:25864; FBst0025864; RRID:BDSC_25864
|
|
| Genetic reagent (D. melanogaster) |
UAS-Synj-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:27489; FBst0027489; RRID:BDSC_27489
|
|
| Genetic reagent (D. melanogaster) |
UAS-Pi3K68D-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:31252; FBst0031252; RRID:BDSC_31252
|
|
| Genetic reagent (D. melanogaster) |
UAS-Pten-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:25967; FBst0025967; RRID:BDSC_25967
|
|
| Genetic reagent (D. melanogaster) |
UAS-Pi3K92E-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:27690; FBst0027690; RRID:BDSC_27690
|
|
| Genetic reagent (D. melanogaster) |
UAS-norpA-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:31197; FBst0031197; RRID:BDSC_31197
|
|
| Genetic reagent (D. melanogaster) |
UAS-fwd-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:31187; FBst0031187; RRID:BDSC_31187
|
|
| Genetic reagent (D. melanogaster) |
UAS-Plc21C-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:31269; FBst0031269; RRID:BDSC_31269
|
|
| Genetic reagent (D. melanogaster) |
UAS-sl-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:32906; FBst0032906; RRID:BDSC_32906
|
|
| Genetic reagent (D. melanogaster) |
UAS-CG3530-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:38340; FBst0038340; RRID:BDSC_38340
|
|
| Genetic reagent (D. melanogaster) |
UAS-CG5026-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:57020; FBst0057020; RRID:BDSC_57020
|
|
| Genetic reagent (D. melanogaster) |
UAS-PIP4K-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:65891; FBst0065891; RRID:BDSC_65891
|
|
| Genetic reagent (D. melanogaster) |
UAS-Sac1-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:56013; FBst0056013; RRID:BDSC_56013
|
|
| Genetic reagent (D. melanogaster) |
UAS-Pi3K59F-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:64011; FBst0064011; RRID:BDSC_64011
|
|
| Genetic reagent (D. melanogaster) |
UAS-Pi3K68D-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:35265; FBst0035265; RRID:BDSC_35265
|
|
| Genetic reagent (D. melanogaster) |
UAS-PIP5K59B-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:62855; FBst0062855; RRID:BDSC_62855
|
|
| Genetic reagent (D. melanogaster) |
UAS-Pi3K93E-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:61182; FBst0061182; RRID:BDSC_61182
|
|
| Genetic reagent (D. melanogaster) |
UAS-mtm-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:57298; FBst0057298; RRID:BDSC_57298
|
|
| Genetic reagent (D. melanogaster) |
UAS-Pi3K21B-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:38991; FBst0038991; RRID:BDSC_38991
|
|
| Genetic reagent (D. melanogaster) |
UAS-Pi3K59F-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:36056; FBst0036056; RRID:BDSC_36056
|
|
| Genetic reagent (D. melanogaster) |
UAS-FIG4-RNAi
|
Bloomington Drosophila Stock Center |
BDSC:58063; FBst0058063; RRID:BDSC_5806335265
|
|
| Cell line (Homo-sapiens) |
HEK293 |
ATCC |
CRL-1573 |
|
| Recombinant DNA reagent |
P4M::GFP (plasmid) |
Hammond et al., 2014
|
|
|
| Recombinant DNA reagent |
P4M × 2::GFP (plasmid) |
Hammond et al., 2014
|
|
|
| Recombinant DNA reagent |
PLC-PH::GFP (plasmid) |
Hammond et al., 2014
|
|
|
| Recombinant DNA reagent |
pGU (plasmid) |
Lu et al., 2021
|
|
|
| Recombinant DNA reagent |
MaLionR (plasmid) |
Addgene |
Addgene #113908 |
|
| Recombinant DNA reagent |
pNP (plasmid) |
Qiao et al., 2018
|
|
|
| Recombinant DNA reagent |
pNP-fwd-KD (plasmid) |
This paper |
|
|
| Recombinant DNA reagent |
pNP-PI4KII-KD (plasmid) |
This paper |
|
|
| Recombinant DNA reagent |
pNP-PI4KIIIα-KD (plasmid) |
This paper |
|
|
| Recombinant DNA reagent |
pNP-PI4K-2KD (plasmid) |
This paper |
|
|
| Recombinant DNA reagent |
pNP-PI4K-3KD (plasmid) |
This paper |
|
|
| Commercial assay or kit |
Plasmid midi kid |
Qiagen |
Cat#12,143 |
|
| Commercial assay or kit |
Plasmid mini kit |
Thermo Scientific |
Cat#K0503 |
|
| Commercial assay or kit |
Gel extraction |
Thermo Scientific |
Cat#K0692 |
|
| Chemical compound, drug |
Halocarbon oil |
Halocarbon 95 oil |
Cat#9002-83-9 |
|
| Chemical compound, drug |
X-treme Gene 9 DNA transfection reagent |
Sigma |
Cat#6365787001 |
|
| Chemical compound, drug |
Fluorescent PM dye |
ThermoFisher |
ThermoFisher, Cat#C10046
|
|
| Chemical compound, drug |
2-Deoxy-D-glucose |
Sigma |
Cat#D8375 |
|
| Chemical compound, drug |
Antimycin A |
Sigma |
Cat#A8674 |
|
| Chemical compound, drug |
Membrane dye |
Invitrogen CellMask |
Cat# C10046
|
|
| Chemical compound, drug |
Wortmannin |
Sigma |
Cat# 19545-26-7 |
|
| Software, algorithm |
Fiji (ImageJ) |
https://imagej.net/Fiji
|
https://imagej.net/Fiji
|
|
| Software, algorithm |
GraphPad Prism 8.0 |
GraphPad Software |
http://www.graphpad.com/
|
|
| Other |
air-permeable membrane |
YSI Inc |
YSI Membrane Model #5,793 |
YSI Inc, Yellow Springs, OH |
