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. 2022 Jun 7;50(2):100. doi: 10.3892/ijmm.2022.5156

Figure 4.

Figure 4

Silencing circITGB1 reverses the inflammatory response by increasing miR-328-3p expression in H/R-exposed HK-2 cells. (A) HK-2 cells were co-transfected with miR-328-3p inhibitor/NC inhibitor and si-circITGB1/si-NC and then exposed to H/R. Cell viability was evaluated using CCK-8 assay by detecting the absorbance at 450 nm. (B and C) Cells were stabled with Annexin V or PI solutions, and cell apoptosis was detected using flow cytometry. (D-F) ELISA of the TNF-α, IL-6 and IL-1β concentrations in HK-2 cells. Data are presented as the mean ± standard deviation from three independent experiments. ***P<0.001 vs. control group; ###P<0.001 vs. H/R + si-NC group; &&P<0.01 and &&&P<0.001 vs. H/R + si-circITGB1 + miR-328-3p inhibitor group. circITGB1, circular RNA integrin beta 1; H/R, hypoxia/reperfusion; IL-1β, interleukin-1β; IL-6, interleukin-6; TNF-α, tumor necrosis factor-α.