Extended Data Fig. 4. (related to Figure 3 a-e) Coomassie stain of affinity-purified ATGL-288 WT and ATGL-288[S47A] mutant enzymes.

(a) Coomassie-stained SDS gel showing induction and purification of ATGL-288 WT enzyme from E. coli Arctic Express. (b) Coomassie-stained SDS gel showing affinity-purified WT ATGL-288 and mutant ATGL-288[S47A] enzymes. Depiction of ATGL-288[S47A] plasmid construct and host e-coli culture (bottom panel).