Extended Data Fig. 5. (related to Figure 3): PG WAT and serum 5-PAHSA, free and TG-esterified OAHSA and POHSA/OAHPA levels are lower in adipose tissue of AT-Atgl-KO mice.
Atgl-KO and littermates Atglflfl female mice (10-11 weeks old) were sacrificed in the ad-lib fed state for experiments in panels (a-n). Endogenous 5-PAHSA levels in (a) PG WAT and (b) serum. Endogenous OAHSA isomers levels in (c) SQ WAT, (d) PG WAT, (e) BAT and (f) liver. Tissue levels of (g) total OAHSA and (h) total POHSA/OAHPA. TG-esterified OAHSA isomer levels in (i) SQ WAT, (j) PG WAT and (k) liver. Tissue levels of (l) total TG-esterified OAHSA and (m) total TG-esterified POHSA/OAHPA. (n) Adipose and liver tissue weights. (n = 5 per genotype for serum samples and 6 for all other tissues, mean ± SEM), *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. FL/FL mice, t-test, two-tailed (a-n). Fewer than 5 samples for panel b or 6 samples for panels a and c-n indicates the other samples were below the limit of quantification for our MS method. ND means not detected.