Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 17;606(7916):1027–1031. doi: 10.1038/s41586-022-04857-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 1 — a, Scheme of HBV infection and MTT cell viability assay for rhNTCP. Free rhNTCP competes for ligands with NTCP expressed on the surface of HepG2-NTCP-C4 cells, so that if more free rhNTCP is added, the HBV infection rate of the cells falls. Myrcludex-B, an inhibitor of HBV infection, was used as a positive control. MTT assay was also performed with the same scheme of HBV assay to determine whether additives such as rhNTCP or Myrcludex-B affect HepG2-NTCP-C4 cell viability. b, Scheme of HBV preS1 attachment assay for rhNTCP. HepG2-NTCP-C4 cells to which preS1 is attached show red fluorescence by the TARMA-preS1 probe, and HepG2-NTCP-C4 cells to which preS1 is not attached show blue fluorescence around the nucleus. Less preS1 attaches to HepG2-NTCP-C4 cells as more rhNTCP is added. c, Scheme of bile acid uptake assay for NTCP. Bile acid uptake was measured in Huh7 cells transfected with the expression plasmid for the wild-type or mutant NTCP. Myrcludex-B was used as a positive control. d, Fluorescence images of HBV infection assay in HepG2-hNTCP-C4 cells. It was confirmed that the HBV infection of HepG2-NTCP-C4 cells decreased as more rhNTCP was added. Fluorescent signals for HBc protein were observed with a fluorescence microscope (BZ-X710, KEYENCE). The micrographs shown were obtained in one of three duplicate experiments, which together with the result of Fig. 1a, support the conclusion that rhNTCP inhibits HBV infection. The scale bar in the control image is 100 µm long.