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. 2022 May 17;606(7916):1027–1031. doi: 10.1038/s41586-022-04857-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a, Phase contrast (left) and fluorescence microscopy image (right) of Sf9 cells expressing NTCP-GFP. Fluorescence indicates level of NTCP-GFP expression. Similar results were obtained in five separate experiments. b, Representative size-exclusion chromatography profile of NTCP (black line) and complex with Fab (blue line). Inset shows SDS–PAGE gel of NTCP and complex with Fab used for cryo-EM grid preparation. SDS-PAGE gels were visualized by Coomassie-blue staining. c, DSF thermal stability assay of NTCP, and NTCP-Fab complex with or without 50 µM TCA. Raw data were plotted with error bars indicating the standard deviations of double samples (left). The T m values were calculated as a graph fitted to the Boltzmann sigmodal equation (right). Binding of one Fab fragment to NTCP show a strong antibody dependent stabilisation of NTCP, raising the melting point from 52.3 ± 0.4 °C to 57.9 ± 0.4 °C.