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. 2022 May 17;606(7916):1027–1031. doi: 10.1038/s41586-022-04857-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a, Schematic flow chart of the classification and refinement procedures to resolve the NTCP-Fab complex structure. b, Representative raw micrograph of NTCP-Fab complex. c, Gallery of two-dimensional class averages, with a window size of 232 Å. d, Final three-dimensional density map coloured by local resolution of the NTCP-Fab complex. e, Euler angle distribution of all particles included in the calculation of the final three-dimensional reconstruction. f, Fourier shell correlation curve of the globally refined NTCP-Fab complex after the post-processing with RELION. The red line intercepts the y axis at a FSC value of 0.143.