Table 2.
Increased lipids in SH-SY5Y cells after the treatment with Dunaliella salina extract at 20 μg/mL (n = 5) in comparison to control conditions (n = 5) after 24 h. Significance is determined using a FC threshold ≥ 1.5 and the Mann–Whitney U test with FDR < 0.05
| Lipid name | Ionization mode | MSI level | FC | p-value | FDR |
|---|---|---|---|---|---|
| TG 58:6 | ESI ( +) | 1 | 1.5317 | 7.94E-03 | 0.014086 |
| TG 52:3 | ESI ( +) | 1 | 1.5887 | 7.94E-03 | 0.014086 |
| TG 56:8; B | ESI ( +) | 2b | 1.6453 | 7.94E-03 | 0.014086 |
| TG 50:3; A | ESI ( +) | 2b | 1.6827 | 7.94E-03 | 0.014086 |
| TG 54:5; B | ESI ( +) | 2b | 1.8438 | 7.94E-03 | 0.014086 |
| TG 50:3; B | ESI ( +) | 1 | 2.0165 | 7.94E-03 | 0.014086 |
| PE 36:3 | ESI (-) | 2b | 2.2474 | 7.94E-03 | 0.016941 |
| PI 38:3|PI 18:0_20:3 | ESI (-) | 2a | 2.289 | 7.94E-03 | 0.016941 |
| PC 35:3 | ESI ( +) | 2b | 2.3779 | 7.94E-03 | 0.014086 |
| TG 53:4 | ESI ( +) | 2b | 2.4063 | 7.94E-03 | 0.014086 |
| TG 54:4 | ESI ( +) | 1 | 2.5237 | 7.94E-03 | 0.014086 |
| PC 36:3; A | ESI ( +) | 2b | 2.6273 | 7.94E-03 | 0.014086 |
| TG 56:6 | ESI ( +) | 1 | 2.7066 | 7.94E-03 | 0.014086 |
| PC 38:5; B | ESI ( +) | 2b | 2.7628 | 7.94E-03 | 0.014086 |
| PE O-36:6 | PE O-16:1_20:5 | ESI (-) | 2a | 3.0815 | 7.94E-03 | 0.016941 |
| TG 58:7 | TG 18:0_18:2_22:5 | ESI ( +) | 2a | 3.106 | 7.94E-03 | 0.014086 |
| FA 20:3 | ESI (-) | 2b | 3.1968 | 7.94E-03 | 0.016941 |
| TG 58:9 | ESI ( +) | 2b | 3.23 | 7.94E-03 | 0.014086 |
| FA 18:3 | ESI (-) | 2b | 3.258 | 7.94E-03 | 0.016941 |
| PC O-38:7 | ESI ( +) | 2a | 3.2866 | 7.94E-03 | 0.014086 |
| FA 20:5 | ESI (-) | 2b | 3.6994 | 7.94E-03 | 0.016941 |
| PC 36:5; D | ESI ( +) | 2b | 4.3572 | 7.94E-03 | 0.014086 |
| PC 36:5; B | ESI (-) | 2b | 4.6587 | 7.94E-03 | 0.016941 |
| PC 34:4 | ESI ( +) | 2b | 5.0593 | 7.94E-03 | 0.014086 |
| PC 38:6; A | ESI ( +) | 2b | 5.3763 | 7.94E-03 | 0.014086 |
| TG 53:5 | ESI ( +) | 2b | 5.6114 | 7.94E-03 | 0.014086 |
| PC p-36:5/PC o-36:6 | ESI ( +) | 2b | 5.6156 | 7.94E-03 | 0.014086 |
| TG 56:7; A | ESI ( +) | 2b | 7.0426 | 7.94E-03 | 0.014086 |
| PC 36:4; B | ESI ( +) | 2b | 7.2773 | 7.94E-03 | 0.014086 |
| TG 54:6; C | ESI ( +) | 2b | 7.7059 | 7.94E-03 | 0.014086 |
| TG 52:4 | ESI ( +) | 1 | 8.8299 | 7.94E-03 | 0.014086 |
| PC 35:4 | ESI (-) | 2b | 9.2395 | 7.94E-03 | 0.016941 |
| FA 20:4 | ESI (-) | 2b | 9.9286 | 7.94E-03 | 0.016941 |
| TG 52:6 | ESI ( +) | 2b | 11.788 | 7.94E-03 | 0.014086 |
| TG 54:5 | TG 18:1_18:1_18:3 | ESI ( +) | 2a | 12.212 | 7.94E-03 | 0.014086 |
| TG 58:10 | ESI ( +) | 2b | 13.217 | 7.94E-03 | 0.014086 |
| PC 34:3; B | ESI ( +) | 2b | 14.584 | 7.94E-03 | 0.014086 |
| TG 54:6; B | ESI ( +) | 1 | 16.637 | 7.94E-03 | 0.014086 |
| TG 56:10 | ESI ( +) | 2b | 18.493 | 7.94E-03 | 0.014086 |
| TG 54:7; B | ESI ( +) | 2b | 20.105 | 7.94E-03 | 0.014086 |
| TG 52:5 | ESI ( +) | 1 | 22.05 | 7.94E-03 | 0.014086 |
| TG 56:8; A | ESI ( +) | 2b | 22.978 | 7.94E-03 | 0.014086 |
| PC 36:4; A | ESI ( +) | 1 | 34.748 | 7.94E-03 | 0.014086 |
| PC 36:5; A | ESI ( +) | 2b | 235.33 | 7.94E-03 | 0.014086 |
FA, fatty acids; PC, phosphatidylcholine; PE, phosphatidylethanolamines; PI, phosphatidylinositol; TG, triglyceride; FC, fold change; FDR, false discovery rate; MSI, Metabolomics Standards Initiative; RT, retention time
MSI level 1: m/z, MS/MS, RT; MSI level 2a: m/z, MS/MS; MSI level 2b: m/z, RT