Fig. 4.

CD4⁺ T cells from the large intestines of adoptively transferred Rag1^−/− mice with colitis kill IECs ex vivo. A Schematic representation of the experiment. R23FR mLN CD4⁺ T cells were adoptively transferred into Rag1^−/− mice, and the mice were fed with or without 0.25 g/L Red 40 in drinking water. Flow-sorted CD4⁺ T cells from the large intestines of Rag1^−/− mice at Day 21 were used as effector cells. B Survival of target epithelial cells after coculture with CD4⁺ T cells was assessed with a CellTiter-Glo Luminescent Cell Viability Assay Kit (effector:target ratio = 4:1). n = 6. Each dot represents one well/condition from a representative of three independent experiments with similar results. Ns, not significant, ***p < 0.001, by nonparametric Mann–Whitney test