Fig. 5. Activated/memory phenotype is acquired by naïve CD8 T cells in MHC class I dependent manner.
a Schematic view of the experimental approach shows that naïve and memory TCM CD8 T cells were sorted from healthy adults then labeled with CTV and CFSE respectively. CFSE-labeled TCM cells were stimulated with anti-CD3/CD28 beads (1:1 ratio). After overnight stimulation, beads were removed by plate magnet followed by incubation with CTV-labeled naïve CD8 T cells at ratio 1:1 and 3:1 ratio (memory:naïve) in the presence of different concentrations of anti-MHC class I antibody. Isotype antibody IgG2a was used as a control (2.5 μg/ml). b Representative flow cytometry plots and c Column graph (individual values) tracking the effect of anti-MHC-I antibody at different concentrations (2.5, 5, 10 μg/ml) on the acquisition of activated/memory phenotype by naïve CD8 T cells compared to isotype control. Ordinary one-way ANOVA Bonferroni multiple comparison test was used *P < 0.05 (n = 4). d Representative flow cytometry contour plots and e- Paired analysis showing the incidence of CTV-labeled naïve CD8 T cells that acquired a memory phenotype in the presence of stimulated TCM CD8 T cells (memory:naïve—3:1 ratio) with (black circle) or without (blue circle) anti-MHC Class I antibody 5 μg/ml. Wilcoxon test (paired nonparametric t test) was used *P < 0.05 (n = 6).