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. 2022 Jun 29;5:633. doi: 10.1038/s42003-022-03569-5

Fig. 5. Examination of the accurate quantification of single EV detection by IFCM.

Fig. 5

Analysis of serial dilutions of 5 double-stained (CFDA-SE & anti-tetraspanin mixture) PPP samples showed a linear correlation between (a) the obtained concentration and (b) Equivalent number of Reference Fluorophores (ERF) of fluorescent detection channels Ch02 (CFSE) and Ch05 (APC) with dilution factor (4-fold).