Table 1.

Criteria for events to be classified as true single EVs by IFCM.

#	Criteria	Reasoning
1	Standardization of SSC signals	Allows estimation of particle sizes
2	Single Spot Fluorescence	Single-particle analysis/no coincidence events
3	Colocalization of fluorophores	Indicating the presence of markers in the same particle/event
4	Signal disappears after Detergent Treatment	Confirmation that detected events are of biological origin
5	Linear correlation with Dilution factor	Single-particle analysis & confirmation that events are biological