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. 2022 Jun 29;13:3760. doi: 10.1038/s41467-022-31526-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Heterogeneous refinement (K = 4) of xKCNQ1-CaM-ML277 data set 1 showed three KCNQ1 classes (see Supplementary Fig. 3). Two classes with similar twist were pooled and refined shown here as class I whereas the third class is shown as class II for clarity. Both classes have different CTD twist degrees between them, and compared to our native and previously studied xKCNQ1-CaM map (PDB-ID: 5VMS), indicating a flexibility in the ML277-induced twist of the coiled-coil CTD. The native structure which was determined in this work is shown in light blue. This twist was also observed for the xKCNQ1-CaM-ML277 data set 2. b The twist of the CTD in the ML277-bound xKCNQ1-CaM (light green) compared to our and previously studied native structures (blue and brown, respectively) shows a displacement of the backbone by ~1 Å at the top of the CTD (Asp526) and due to the propagated twist up to ~3 Å around the last modeled residue, Leu556.