Fig. 8.

Inhibition of p38 activity in human DCs promotes Th2-cell differentiation. a RNA analysis of IL4, IL5, IL13, IL17 and IFNG in human blood CD4⁺ T cells activated with human mo-DCs pretreated with HDM in the presence of vehicle or SB203580 (n = 3). b Expression of IL12P40 in human mo-DCs stimulated with HDM in the presence of vehicle or SB203580 for 5 h (n = 3). c Intracellular staining analysis of IL-12p40 expression in AR patient-derived DCs treated with vehicle or SB203580. d Proposed model of the involvement of DC p38α signaling in the regulation of Th2-mediated allergic inflammation. *P < 0.05; **P < 0.01; ns, not significant. Data are pooled from three independent experiments (a) or representative of three independent experiments (b). Student’s t test (a and b) was performed, and the data are presented as the mean  ± SEM