Fig. 4.

Aldehydic load after e-cigarette aerosol or air exposure. A. Representative blot of protein carbonyl expression. B. Quantification of protein carbonylation (n=6/group). C. Free malondialdehyde (MDA) in heart homogenates. The concentration of MDA was normalized by the total amount of protein (n=6/group). D. Representative blot of 4-HNE-induced protein adducts with GAPDH (36 kDa) as a loading control. E. Quantification of 4-HNE-induced protein adducts (n=6/group). F: phospho- and total nuclear factor kappa B (NF-κB, 65 kDa) in heart homogenates (n=6/group). ^p < 0.05, between genotypes; and ⁺p < 0.05, ^&p < 0.01, *p < 0.001, within genotypes, respectively, calculated by one-way ANOVA with Bonferroni correction.