Fig. 4.
Deletion of IFT20 in MSCs promotes adipogenic differentiation but inhibits osteogenic differentiation. (A) Representative image of ALP (Day 5) staining of MSCs from Prx1-Cre; IFT20f/f and littermate control mice. (B) Representative image of ARS (Day 14) staining of MSCs from Prx1-Cre; IFT20f/f and littermate control mice. Relative OD of ARS staining was at right. (C) ALP activity. (D) Representative image of Oil red O staining after adipogenic incubation of 14 days. The corresponding quantitative analyses of Oil red O staining were performed at right. (E, F) Osteogenic and adipogenic markers analyses by qRT-PCR after stimulation with osteogenic or adipogenic media for 14 days as indicated. Error bars were the means ± SEM from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)