Table 1.
Expression of nidogen isoforms in lineage differentiation of musculoskeletal tissues.
| Tissue/cells | Nidogen-1 | Nidogen-2 | Ref. |
|---|---|---|---|
| Cartilage | mouse femoral head (qPCR, IHC) | bovine MCP (IGEM, IHC); mouse femoral head (qPCR, IHC) |
78 |
| mouse TMJ (IHC) | mouse TMJ (IHC) | 80 | |
| mouse embryo (IF) | mouse embryo (IF) | 27 | |
| human embryo metacarpus or rib anlagen (IHC); human healthy and OA knee (IGS, IHC) | human embryo metacarpus or rib anlagen (IHC); human healthy and OA knee (IGS, IHC) | 79 | |
| Fat | human visceral fat (proteomics) | human visceral fat (proteomics) | 54 |
| – | human visceral fat (CILAIR-based secretome analysis) | 62 | |
| mouse epidydimal and subcutaneous fat (IF) | – | 64 | |
| Muscle | mouse embryo limb (IF) | – | 85 |
| mouse diaphragm and limb muscles (IF) | mouse diaphragm and limb muscles (IF) | 84 | |
| mouse cardiac muscles (IF) | mouse cardiac muscles (IF) | 38 | |
| mouse soleus and cardiac muscles (IF, WB) | mouse soleus and cardiac muscles (IF, WB); mouse soleus muscles (IGS) | 36 | |
| mouse skeletal and cardiac muscles (IF, RIA) | mouse skeletal and cardiac muscles (IF, RIA) | 26 | |
| mouse skeletal muscles (IF, NB, RIA, WB) | mouse skeletal muscles (IF, NB, RIA, WB) | 37 | |
| Nerve | C. elegans sublateral nerve and nerve ring (IF) | – | 43 |
| C. elegans nerve cord (IF) | – | 45 | |
| rat DRG (IF) | – | 92 | |
| rat sciatic nerve (IF, ISH, qPCR, WB) | rat sciatic nerve (IF, ISH, qPCR, WB) | 94 | |
| Tendon | mouse (IF) | – | 129 |
| Vessel | mouse embryo radial glia (IF) | – | 135 |
| mouse capillary in muscles (IF) | – | 136 | |
| mouse vessels in RIP-Tag2 pancreatic islet tumors (IF) | – | 67 | |
| mouse retinal capillaries (IF, WB) | mouse retinal capillaries (IF) | 75 | |
| BMSC | expression in human cells following 28-day adipogenic induction (IF) | – | 63 |
| increased expression in human cells following 28-day adipogenic induction (qPCR) | – | 59 | |
| 3T3-L1 | increased expression in supernatants but decreased in mouse cells in 6-day adipogenic induction (IP) | – | 55 |
| – | increased expression in mouse cells in 14-day adipogenesis (microarray) | 56 | |
| peak expression at day 3 in supernatants of mouse cells in 7-day adipogenic induction (SILAC) | peak expression at day 3 in supernatants of mouse cells in 7-day adipogenic induction (SILAC) | 58 | |
| increased expression in supernatants of mouse cells in 9-day adipogenic induction (proteomics) | – | 57 | |
| – | increased in mouse cells in 14-day adipogenic induction (IF, qPCR) | 61 | |
| peak expression in supernatants of mouse cells in the middle stage of 10-day adipogenic induction (MS) | – | 60 |
Abbreviations: BMSC: bone marrow stromal cells; C. elegans: Caenorhabditis elegans; CILAIR: Comparison of Isotope-Labeled Amino acid Incorporation Rates; DRG: dorsal root ganglia; IF: immunofluorescence; IGEM: immunogold electron microscopy; IGS: immunogold histochemistry; IHC: immunohistochemistry; IP: immunoprecipitation; ISH: in situ hybridization; MCP: metacarpophalangeal joint; MS: mass spectrometry; NB: northern blot; OA: osteoarthritis; qPCR: quantitative polymerase chain reaction; RIA: radioimmunoassays; SILAC: stable isotope labeling with amino acids in cell culture; TMJ: temporomandibular joint; WB: western blot.