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. 2020 Nov 21;9(3):741–752. doi: 10.1016/j.gendis.2020.11.009

Figure 4.

Figure 4

YAP1 modulates the motility of 143B cells. (A–C). The 143B cells were transfected with lentiviral vector targeting YAP1 and its control (shNT). The knockdown efficacy of YAP1 was evaluated through real-time PCR (A) and western blots (B, C). GAPDH was used as a reference. shYAP1-1 (recorded as shYAP1) was chosen for the following experiment. (D, E). The migration of 143B cells with YAP1 knockdown was detected by a wound healing assay (24 h). Scale bars: 200 μm. (F–H). The migration (16 h) and invasion (24 h) of 143B cells with YAP1 knockdown was detected by Transwell assay. Scale bars: 200 μm. (I, J). The expression of E-Cadherin, N-Cadherin, Vimentin and MMP2 was evaluated by western blots. GAPDH was used as a reference. The bars show the mean ± SD of three independent experiments.