FIGURE 2.

Glomerular and Tubular Histological Alterations are more Prominent in N vs. J Substrain, and are Correlative with Albuminuria. (A–D) Histologic evaluation of kidneys stained with periodic acid-Schiff method (scale bar = 20 μm). Representative images showing (i) glomerular and (ii) tubular histology from (A,B) C57BL/6 N control and ADR-injected mice, and (C,D), C57BL/6 J control and ADR-injected mice. (Bi and Di) Glomerular damage is indicated with an arrow for a sclerotic segment and circle for hypertrophied podocytes that contain protein reabsorption droplets. (Bii and Dii) Tubular protein casts are indicated with asterisks (*). (E i) Correlation of glomerular segmental sclerosis and podocyte hypertrophy with albuminuria in J and N ADR-injected mice. Segmental sclerosis is indicated with a purple solid line, and the data points are marked as X’s (Blue = N, Green = J). Podocyte hypertrophy is indicated with a dashed line, and the data points are marked as circles (Blue = N, Green = J). (E ii) Correlation of tubular injury (TI) scores with albuminuria in J and N ADR-injected mice (Blue = N, Green = J). The tubular injury was scored subjectively with 0 = normal, 1 = mild, 2 = moderate, 3 = severe.