TABLE 1.
Histological Scoring of ADR-Induced Nephropathy in both N and J Substrains.
| ID | Glomerular | Tubular | |||||
|---|---|---|---|---|---|---|---|
| % Segmental sclerosis | Score | % Global sclerosis | % Podocyte hypertrophy | % Histologically normal | Qualitative assessment of damage | Score | |
| C57BL/6 N Control | |||||||
| #15 | 0 | 0 | 0 | 1 | 99 | Normal | 0 |
| #16 | 0 | 0 | 0 | 3 | 97 | Normal | 0 |
| #17 | 0 | 0 | 0 | 2 | 98 | Normal | 0 |
| C57BL/6 N ADR | |||||||
| #5 | 42 | 3 | 1 | 27 | 30 | Severe multifocal protein casts; some regions have tubular basophilia | 3 |
| #6 | 23 | 2 | 1 | 25 | 51 | Moderate multifocal protein casts; some regions have tubular basophilia | 2 |
| #7 | 41 | 3 | 1 | 30 | 28 | Moderate multifocal protein casts; some regions have tubular basophilia | 2 |
| #8 | 11 | 1 | 1 | 23 | 65 | Mild multifocal protein casts; some regions have tubular basophilia | 1 |
| C57BL/6 J Control | |||||||
| #9 | 0 | 0 | 0 | 8 | 92 | Normal | 0 |
| #10 | 0 | 0 | 0 | 5 | 95 | Normal | 0 |
| #11 | 1 | 0 | 0 | 6 | 93 | Normal | 0 |
| #12 | 0 | 0 | 0 | 9 | 91 | Normal | 0 |
| C57BL/6 J ADR | |||||||
| #1 | 3 | 0 | 0 | 4 | 93 | Mild multifocal tubular basophilia with protein casts | 1 |
| #2 | 3 | 0 | 0 | 3 | 94 | Normal | 0 |
| #3 | 14 | 1 | 0 | 9 | 77 | Mild multifocal protein casts | 1 |
| #4 | 1 | 0 | 0 | 4 | 95 | Normal | 0 |
Podocyte lesions included cytoplasmic vacuolation, protein reabsorption droplets and synechiae. The tubulointerstitium was semi-quantitively scored for tubular dilation, epithelial basophilia and tubular protein casts. The severity score was based on the portion of the tissue section that was affected. Normal samples received a score of 0. Mildly affected samples had approximately 1/3 of their parenchyma with lesions and they received a score of 1; moderate scores (2) were given when between 1/3 and 2/3 of the renal parenchyma had lesions; severely affected kidneys (3) had more than 2/3 of their parenchyma with lesions. Kidney scoring sheet of 100 glomeruli per sample. Scoring for sclerosis is based on <5% = 0, 5%–20% = 1, 20%–40% = 2, and >40% = 3 (Wharram et al., 2005). Tubular injury was assessed subjectively and the scoring system is 0 = normal, 1-mild, 2 = moderate, 3 = severe.