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. 2022 Jun 16;14:902525. doi: 10.3389/fnagi.2022.902525

FIGURE 1.

FIGURE 1

PS deficiency increased oxidative stress in PS-DK fibroblasts. (A) Confocal microscopy of DHE staining. Wild-type and PS-DK fibroblasts were incubated with DHE (5 μM) for 30 min in DMEM containing 10% FBS at 37°C in 5% CO2. The number of DHE-positive cells increased in PS-DK fibroblasts, indicating that presenilin deficiency enhanced ROS production. (B) Ratio of DHE-positive cells in wild-type and PS-DK fibroblasts. n = 6 images from 3 independent experiments, ****P< 0.0001, unpaired two-tailed Student’s t-test. (C) Confocal microscopy of DHE staining after H2O2 treatment. Wild-type and PS-DK fibroblasts were treated with 1 mM H2O2 for 30 min and then stained with DHE (5 μM). (D) Fluorescence intensity in wild-type and PS-DK fibroblasts were determined Image J software. n = 10 images from 3 independent experiments, ****P< 0.0001, unpaired two-tailed Student’s t-test. Scale bars, 20 μm. All quantitative data shown as mean±SEM.