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. 2022 Jun 16;14:902525. doi: 10.3389/fnagi.2022.902525

FIGURE 2.

FIGURE 2

PS-DK fibroblasts generated more ROS by iron-treatment and showed lower intracellular ferritin levels than wild-type fibroblasts. (A) ROS fluorescence density were measured 16 h after treatment with ferrous sulfate. PS-DK fibroblasts showed higher ROS levels after iron-treatment. n = 3, from 3 independent experiments, *P< 0.05, unpaired two-tailed Student’s t-test. (B) Western blot analysis of intracellular ferritin light and heavy chains, PS1 C-terminal fragment (CTF) and PS2 CTF. (C,D) Quantification of intracellular ferritin light and heavy chain levels. Their levels were normalized to α-tubulin protein levels. n = 3, from 3 independent experiments, *P< 0.05, **P< 0.01, unpaired two-tailed Student’s t-test. All quantitative data shown as mean±SEM. Ferritin-L, ferritin light chain. Ferritin-H, ferritin heavy chain.