FIGURE 3.

PS deficiency reduced ferritin levels in response to iron treatment. (A) Western blot analysis of ferritin light chain in wild-type and PS-DK fibroblasts treated with ferrous sulfate. (B) Quantification of intracellular ferritin light chain levels. Their levels were normalized to α-tubulin protein levels. n = 3, from 3 independent experiments, *P< 0.05, ^**P< 0.01, ^***P< 0.001, one-way ANOVA followed by Tukey’s multiple-comparison tests. (C) Western blot analysis of ferritin heavy chain in wild-type and PS-DK fibroblasts treated with ferrous sulfate. (D) Quantification of intracellular ferritin heavy chain levels. Their levels were normalized to α-tubulin protein levels. n = 3, from 3 independent experiments, ^**P< 0.01, ^***P< 0.001, one-way ANOVA followed by Tukey’s multiple-comparison tests. All quantitative data shown as mean ± SEM. Ferritin-L, ferritin light chain. Ferritin-H, ferritin heavy chain.