Figure 1.

Cell-free fat extract (CEFFE) improves ovarian function in aged mice. (A) The body weight of mice was monitored every two days during the treatment duration (n=5). (B) Gross morphologies of dissected ovaries from different groups (n=3). (C) The index of ovaries (the ratio of ovarian weight to body weight) in each group were compared and analyzed (n=5). (D–F) Serum AMH, E₂, and FSH secretion levels were measured via ELISA every two weeks until 8 weeks after treatment (n=3-5). (G) Representative ovary morphology (a) and follicle morphology (b) of Hematoxylin and Eosin staining from each group were captured. Scale bar=200 μm or 50 μm. (H) The number of different stage follicles was calculated and analyzed (n=7). Pri, primordial follicles; Prim, primary follicles; Sec, secondary follicles; Ant, Antral follicles; Atr, atretic follicles; Total, The total number of health follicles (including primordial, primary, secondary and antral follicles). Data are represented as mean ± SD, ns: P > 0.05, ^* P < 0.05, ^** P < 0.01, ^*** P < 0.001, and ^**** P < 0.0001.