Figure 5.

CEFFE recovers proliferation and E₂ secretion of senescent KGN cells. (A) Proliferation of KGN cells was measured via EdU assay. Proliferative cells were labeled via green fluorescence, and all nuclei were labeled via blue fluorescence. Scale bar=50 μm. (B) Statistics of proliferative cell rates. (C) Proliferation of KGN cells was measured using the CCK-8 assay at 24 h, 48 h, and 72 h. (D) The E₂ content in cellular supernatant was measured via ELISA, and the results were standardized with the concentration of KGN cell proteins in each group. Three independent experiments were conducted in each group, and data are represented as the mean ± SD, ns: P > 0.05, ^* P < 0.05, ^** P < 0.01, ^**** P < 0.0001, ^####P < 0.0001 ^*Compared with Aged group, ^#Compared with Control group.