FIGURE 1.

Fisetin prevented reduction of cell viability by H₂O₂ treatment in ARPE-19 cells. Cells were treated with different concentrations of fisetin (A) or H₂O₂ (B) for 24 h, or fisetin was pretreated for 1 h and then treated with H₂O₂ for an additional 24 h (C), followed by the CCK8 assay. Fisetin have no cytotoxicity up to 20 μM, and H₂O₂ result in reduction in cell viability in a dose-dependently. The reduction in cell viability by H₂O₂ gradually improved with increasing fisetin pretreatment concentration. Data are graphically presented as mean ± SD (n = 4, ^** p ˂ 0.01 and ^*** p ˂ 0.001, compared with the control group; ^## p ˂ 0.01 compared with H₂O₂ treatment group).