FIGURE 2.

Fisetin attenuated H₂O₂-induced intracellular ROS generation in ARPE-19 cells. Cells were treated with or without 20 μM fisetin for 1 h and then treated with 500 μM H₂O₂ for 1 h. The levels of ROS production were determined using DCF-DA dye. (A) Representative results from flow cytometry analyses are shown. (B) The values of flow cytometry results are expressed as mean ± SD (n = 3, ^* p ˂ 0.05, and ^*** p ˂ 0.001 compared with the control group; ^### p ˂ 0.001 compared with H₂O₂ treatment group). (C) The level of ROS (green) was observed under a fluorescence microscope, and representative images are presented. Nuclei were counterstained with DAPI (blue). Pretreatment of fisetin significantly suppressed increment of DCF-positive cell population following H₂O₂.