Figure 1.
Constitutive elements analysis of Addgene most commonly used AAV vectors
Number of occurrences of the indicated elements among Addgene’s top plasmids sorted by size (base pairs, bp).
(A) Backbones. The dotted line shows the 4.7 kb payload limit.
(B) Promoters. WPRE and polyA sequences.
(C) Distribution of backbones’ sizes for recombinase-inducible plasmids from Addgene. Each row represents specific recombinase dependencies as described on the left y axis, and its corresponding VTK constructs are represented on the right y axis.
(D) Number of occurrences of backbones in direct comparison to Cre-ON conformation.
In (A–D), the colored dots on the x axis indicate the size of the corresponding elements used in VTK vectors. Green dots for common elements to all VTKs (WPRE and polyA), purple dots for VTKS1–6, and yellow dots for VTKD1–6.