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. 2022 Jun 13;2(6):100238. doi: 10.1016/j.crmeth.2022.100238

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Characterization of latently and productively infected CD4⁺ T cell populations using CyTOF

(A) pMorpheus-V5-infected primary human CD4⁺ T cells stimulated with IL-2 or activated with IL-2/α-CD3/CD28 beads were analyzed using CyTOF at 5 dpi. Latently (V5_POS, NGFR_POS; yellow) or productively (HSA_POS, mCherry_POS, V5_POS, NGFR_POS, p24_POS; green) infected cells were identified. Data for each donor (±SD) are indicated by a specific symbol.

(B) The bar graph shows the fold change of latently or productively infected cells depending on cellular activation. The average of two individual donors is shown.

(C) The proportion of latently or productively infected CD4⁺ T cells stimulated with IL-2 for three days. The average of two individual donors is shown with the percentage and the total number of cells.

(D) The proportion of latently or productively infected CD4⁺ T cells stimulated with IL-2 and α-CD3/CD28. The average of two individual donors is shown with the percentage and the total number of cells.

(E) viSNE analysis of pMorpheus-V5-infected CD4⁺ T cells stimulated with IL-2 alone or with IL-2 and α-CD3/CD28. The tSNE1 and tSNE2 axes are based on the relevant markers of the subsets defined by the Cytobank program. The results are based on 66,000 cells for each condition from each donor identified by the circle and triangle symbols in (A).